X-ray CT (computed tomography) is a non-destructive 3D imaging technique. It provides fast, high-resolution imaging with little to none of the extensive sample preparation required by other imaging techniques, such as electron microscopy. It is a powerful technique to reveal internal structures of life science samples, such as animals, plants, and insects. Moreover, X-ray CT experiments can be performed in situ and in vivo for these types of samples. The recent development of various contrast-enhancing techniques has extended the capability of X-ray CT (Rawson et al., (2020) BMC Biology, 18:21).